CRISPR Cas9: Making Progress Against Cancer – Abstract

Mechanism of action of CRISPR Cas9 tool

Journal of Environmental and Agricultural Sciences (JEAS). Akbar et al., 2020. Volume 22(2): 44-57

Open Access – Review Article

CRISPR Cas9: Making Progress Against Cancer

Ghulam Akbar 1,*, Muhammad Anjum Zia 1, Faiz Ahmad Joyia 2, Ali Ahmad 3, Sana Ahmad 1,
Neha Arooj 1, Muhammad Muzammal Saeed 1
1 Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan

2 Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad, Pakistan
3 Department of Agronomy, University of Agriculture, Faisalabad, Pakistan

Abstract: CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat) is one of the most potent methods to genome editing for different organisms. As part of the adaptive immune system of bacteria, CRISPR/Cas9 and its modified versions are used globally in genome engineering to stimulate or suppress the expression of the genes. CRISPR/Cas9 also promises to revolutionize cancer research as it is an efficient technique to get the insights of tumorigenesis, identify developmental drug targets, and arm cell therapies. Currently used applications of CRISPR/Cas9 technology for cancer therapy are reviewed here. In this review, we have enlisted and discussed the impact of CRISPR/Cas9 in creating organoid and mouse models of cancer. Moreover, this review also describes CRISPR Cas9 versatility, in vivo delivery system, drug efficacy, different CRISPR systems, in vivo genome editing, and target discovery.

Keywords: Versatility, cancer model, target delivery, industrial perspectives, innate immunity, RNA interference, gene therapy.

*Corresponding author: Ghulam Akbar:

Cite this article as: Akbar, G., M.A. Zia, F.A. Joyia, A. Ahmad, S. Ahmad, N. Arooj and M.M. Saeed. 2020. CRISPR Cas9: Making progress against cancer. Journal of Environmental& Agricultural Sciences. 22(2): 44-57.

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Copyright © Akbar et al., 2020. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium provided the original author and source are appropriately cited and credited.

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