Journal of Environmental and Agricultural Sciences (JEAS). Akbaba et al., 2023. 25(1&2):xx-xx.
Open Access – Research Article
Health-Protecting Properties of Olive Leaves (Olea europaea L.) via Antioxidant Profile and Phenolic Composition
Department of Medical Biology, Faculty of Medicine, Kirikkale University, 71450, Kirikkale, Turkiye
Abstract: Olive (Olea europaea L.) leaves are major by-product of the olive oil industry, containing a variety of bioactive chemicals with significant potential health benefits. The major components of olives responsible for their therapeutic properties are phenolic compounds. This study aimed to determine the total phenolic content, total flavonoid content, and antioxidant activity of methanolic extracts of olive leaves obtained using two techniques: maceration and microwave-assisted extraction. The antioxidant activities of the extracts were determined via ferric-reducing antioxidant power and phenanthroline-metal chelating capacity assays. The extract obtained via maceration yielded 37 mg g-1 of total phenolics, 16 mg g-1 total of flavonoids, 50 mg g-1 ferric-reducing antioxidant biomolecules, and 140 mg g-1 of phenanthroline metal chelating phytochemicals. However, microwave-assisted extraction yielded 38 mg g-1 of total phenolics, 21 mg g-1 of total flavonoids, 52 mg g-1 of ferric-reducing antioxidant biomolecules, and 142 mg g-1 of phenanthroline-metal chelating phytochemicals. In comparison to the maceration, the microwave-assisted extraction method resulted in slightly higher quantities of bioactive compounds in a relatively short time, while using less solvent. This study showed that olive leaves have great potential for bioactive molecules and antioxidant profiles. This study also reports the importance of taking advantage of olive leaves, which are byproducts of factories and are generally considered waste. Therefore, olive leaves are highly recommended in the food industry as natural antioxidants rather than synthetic antioxidants, which can cause a variety of health problems.
Keywords: Microwave-assisted extraction, phenolic compounds, Olea europaea, antioxidant potential, olive.
*Corresponding author: Emel Akbaba, firstname.lastname@example.org
Cite this article as:
Akbaba, E. 2023. Health-protecting properties of olive leaves (Olea europaea L.) via antioxidant profile and phenolic composition. Journal of Environmental & Agricultural Sciences. 25(1&2): XX. [Abstract] [View Full-Text] [Citations]
Copyright © Akbaba, 2023 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium provided the original author and source are appropriately cited and credited.
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Akbaba, E. 2021. Characterization of bioactive and antioxidant composition of mountain tea (Sideritis montana ssp. montana): Microwave-assisted technology. Int. J. Second. Metabol. 8: 159-171.
Altıok, E., D. Bayçın, O. Bayraktar and S. Ülkü, 2008. Isolation of polyphenols from the extracts of olive leaves (Olea europaea L.) by adsorption on silk fibroin. Separation and Purification Technol. 62: 342-348.
Barazani, O., A. Dag and Z. Dunseth. 2023. The history of olive cultivation in the southern Levant. Front. Plant Sci. 14: 1131557 .
Berker, K.I., K. Güçlü, İ. Tor, B. Demirata and R. Apak. 2010. Total Antioxidant Capacity Assay Using Optimized Ferricyanide/Prussian Blue Method. Food Analytical Methods. 3: 154-168.
Bilgin, M. and S. Şahin. 2013. Effects of geographical origin and extraction methods on total phenolic yield of olive tree (Olea europaea) leaves. J. Taiwan Inst. Chemical Eng. 44: 8-12.
Brandt, W. W., F. P. Dwyer and E. D. Gyarfas. 1954. Chelate complexes of 1,10-phenanthroline and related compounds. Chem. Rev. 54: 959-1017.
Caponio, F., G. Difonzo, M. Calasso, L. Cosmai and M. De Angelis. 2019. Effects of olive leaf extract addition on fermentative and oxidative processes of table olives and their nutritional properties. Food Res. Int. 116: 1306-1317.
Cedola, A., C. Palermo, D. Centonze, M. A. Del Nobile and A. Conte. 2020. Characterization and bio-accessibility evaluation of olive leaf extract-enriched “Taralli”. Foods. 9: 1268.
Chiou, A., F. N. Salta, N. Kalogeropoulos, A. Mylona, I. Ntalla and N. K. Andrikopoulos. 2007. Retention and distribution of polyphenols after pan-frying of french fries in oils enriched with olive leaf extract. J. Food Sci. 72: S574-S584.
FAO. 2021. “Http://Www.Fao.Org/Faostat/En/#data/QCL/Visualize.” 2021.
Giacometti, J., S. Milovanović, D. Jurc\̌ić Momc\̌ilović and M. Bubonja-S\̌onje. 2021. Evaluation of antioxidant activity of olive leaf extract obtained by ultrasound-assisted extraction and their antimicrobial activity against bacterial pathogens from food. Int. J. Food Sci. Technol. 56: 4843-4850.
González-Burgos, E., M. E. Carretero and M. P. Gómez-Serranillos. 2011. Sideritis spp.: Uses, chemical composition and pharmacological activities—A review. J. Ethnopharmacol. 135: 209-225.
Guglielmotti, M., P. Passaghe and S. Buiatti. 2020. Use of olive (Olea europaea L.) leaves as beer ingredient, and their influence on beer chemical composition and antioxidant activity. J. Food Sci. 85: 2278-2285.
Gupta, P., and B. De. 2017. Differential responses of cell wall bound phenolic compounds in sensitive and tolerant varieties of rice in response to salinity. Plant Signal. Behav. 12 (10): e1379643.
Hayes, J. E., P. Allen, N. runton, M.N. ’Grady and P. Kerry. 2011. Phenolic composition and in vitro antioxidant capacity of four commercial phytochemical products: olive leaf extract (Olea europaea L.), lutein, sesamol and ellagic acid. Food Chem. 126 (3): 948–55.
Herrero, M., T. N. Temirzoda, A. Segura-Carretero, R. Quirantes, M. Plaza and E. Ibañez. 2011. New possibilities for the valorization of olive oil by-products. J. Chromatog. A. 1218: 7511-7520.
Irakli, M., P. Chatzopoulou and L. Ekateriniadou. 2018. Optimization of ultrasound-assisted extraction of phenolic compounds: Oleuropein, phenolic acids, phenolic alcohols and flavonoids from olive leaves and evaluation of its antioxidant activities. Ind. Crops Prod. 124: 382-388.
Ismail, M. A., M. N. Norhayati and N. Mohamad. 2021. Olive leaf extract effect on cardiometabolic profile among adults with prehypertension and hypertension: a systematic review and meta-analysis. PeerJ. 9: e11173.
Kashaninejad, M., M. T. Sanz, B. Blanco, S. Beltrán and S. M. Niknam. 2020. Freeze dried extract from olive leaves: Valorisation, extraction kinetics and extract characterization. Food Bioprod. Process. 124: 196-207.
Khemakhem, I., A. Fuentes, M. J. Lerma-García, M. A. Ayadi, M. Bouaziz and J. M. Barat. 2019. Olive leaf extracts for shelf life extension of salmon burgers. Food Sci. Technol. Int. 25: 91-100.
Khwaldia, K., N. Attour, J. Matthes, L. Beck and M. Schmid. 2022. Olive byproducts and their bioactive compounds as a valuable source for food packaging applications. Comprehensive Rev. Food Sci. Food Saf. 21: 1218-1253.
Kranz, P., N. Braun, N. Schulze and B. Kunz. 2010. Sensory quality of functional beverages: bitterness perception and bitter masking of olive leaf extract fortified fruit smoothies. J. Food Sci. 75: S308-S311.
Lama-Muñoz, A., M. d. M. Contreras, F. Espínola, M. Moya, I. Romero and E. Castro. 2020. Content of phenolic compounds and mannitol in olive leaves extracts from six Spanish cultivars: Extraction with the Soxhlet method and pressurized liquids. Food Chem. 320: 126626.
Losada-Echeberría, M., A. Taamalli, V. Micol, M. Herranz-López, D. Arráez-Román and E. Barrajón-Catalán. 2017. P 215 – Antioxidant enriched olive leaf extracts show antiproliferative activity in cellular models of breast cancer. Free Radic. Biol. Med. 108: S92.
Magalhães, L. M., M. A. Segundo, S. Reis and J. L. F. C. Lima. 2008. Methodological aspects about in vitro evaluation of antioxidant properties. Anal. Chim. Acta. 613: 1-19.
Marchev, A. S., L. V. Vasileva, K. M. Amirova, M. S. Savova, I. K. Koycheva, Z. P. Balcheva-Sivenova, S. M. Vasileva and M. I. Georgiev. 2021. Rosmarinic acid – From bench to valuable applications in food industry. Trend. Food Sci. Technol. 117: 182-193.
Markhali, F. S., J. A. Teixeira and C. M. R. Rocha. 2020. Olive tree leaves—a source of valuable active compounds. Processes. 8: 1177.
Marković, M., E. Mezzatesta, S. Porcier, C. Vieillescazes and C. Mathe. 2022. Rethinking the process of animal mummification in ancient Egypt: Molecular Characterization of embalming material and the use of brassicaceae seed oil in the mummification of Gazelle Mummies from Kom Mereh, Egypt. Molecules. 27: 1532.
Martín-García, B., S. Pimentel-Moral, A.M. Gómez-Caravaca, D. Arráez-Román and A. Segura-Carretero. 2020. Box-Behnken experimental design for a green extraction method of phenolic compounds from olive leaves. Ind. Crops Prod. 154: 112741.
Medina, E., C. Romero, P. Garcia and M. Brenes. 2019. Characterization of bioactive compounds in commercial olive leaf extracts, and olive leaves and their infusions. Food Function. 10 (8): 4716–24.
Monteleone, J.I., E. Sperlinga, L. Siracusa, G. Spagna, L. Parafati, A. Todaro and R. Palmeri. 2021. Water as a solvent of election for obtaining oleuropein-rich extracts from olive (Olea europaea) leaves. Agronomy. 11 (3): 465.
Ozturk, M., V. Altay, T.M. Gönenç, B.T. Unal, R. Efe, E. Akçiçek and A. Bukhari 2021. an overview of olive cultivation in Turkey: botanical features, eco-physiology and phytochemical aspects. Agronomy. 11(2): 295.
Paiva-Martins, F., R. Correia, S. Félix, P. Ferreira and M.H. Gordon. 2007. Effects of enrichment of refined olive oil with phenolic compounds from olive leaves. J. Agric. Food Chem. 55 (10): 4139–43.
Perron, N.R., and J.L. Brumaghim. 2009. A Review of the antioxidant mechanisms of polyphenol compounds related to ıron binding. Cell Biochem. Biophys. 53 (2): 75–100.
Rosa, G.S., Martiny, T.R., Dotto, G.L., Vanga, S.K., Parrine, D., Gariepy, Y., Lefsrud, M., and Raghavan, V. 2021. Eco-Friendly extraction for the recovery of bioactive compounds from Brazilian olive leaves. Sustain. Mat. Technol. 28: e00276.
Salem, M.B., H. Affes, K. Ksouda, Z. Sahnoun, K.M. Zeghal and S. Hammami. 2015. Pharmacological activities of Olea europaea leaves. J. Food Process. Rreserv. 39 (6): 3128–36
Sanchez de Medina, V., Priego-Capote, F., Jiménez-Ot, C., and Luque de Castro, M.D. 2011. Quality and stability of edible oils enriched with hydrophilic antioxidants from the olive tree: The role of enrichment extracts and lipid composition. J. Agric. Food Chem. 59 (21): 11432–41.
Silva, A.F.R., D. Resende, M. Monteiro, M.A. Coimbra, A.M.S. Silva and S.M. Cardoso. 2020. Application of hydroxytyrosol in the functional foods field: From ingredient to dietary supplements. Antioxidants. 9 (12): 1–23.
Šimat, V., D. Skroza, G. Tabanelli, M. Čagalj, F. Pasini, A. M. Gómez-Caravaca, C. Fernández-Fernández, M. Sterniša, S. Smole Možina, Y. Ozogul and I. Generalić Mekinić. 2022. Antioxidant and antimicrobial activity of hydroethanolic leaf extracts from six Mediterranean olive cultivars. Antioxidants 11: 1656.
Singleton, V.L., R. Orthofer and M.B.T. Rosa. 1999. Methods in enzymology lamuela-raventós. analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu Reagent. Methods Enzymol. 299: 178–84.
Sofo, A., S. Manfreda, M. Fiorentino, B. Dichio and C. Xiloyannis. 2008. The Olive Tree: A Paradigm for Drought Tolerance in Mediterranean Climates. Hydrol. Earth Syst. Sci. 12 (1): 293–301.
Soler-Rivas, C., J.C. Espin and H.J. Wichers. 2000. Oleuropein and Related Compounds. J. Sci. Food Agric. 80 (7): 1013–23.
Szydłowska-Czerniak, A., C. Dianoczki, K. Recseg, G. Karlovits and E. Szłyk. 2008. Determination of antioxidant capacities of vegetable oils by ferric-ion spectrophotometric methods. Talanta. 76 (4): 899–905.
Trifa, W., S. Akkal, M. Lefahal, L. Benmekhebi and S. Khennouf. 2020. Preliminary screening of extracts for determination of antioxidant activity by different methods. Curr. Iss. Pharm. Med. Sci. 33 (1): 32–37.
Tsimidou, M.Z., and Papoti, V.T. 2010. Chapter 39 – Bioactive Ingredients in Olive Leaves. In Olives and Olive Oil in Health and Disease Prevention, edited by V.R. Preedy and R. Ross B T – Olives and Olive Oil in Health and Disease Prevention Watson, p: 349–56.
Vezza, T., F. Algieri, A. Rodriguez-Nogales, J. Garrido-Mesa, M.P. Utrilla, N. Talhaoui, A.M. Gomez-Caravaca, et al. 2017. Immunomodulatory properties of Olea europaea leaf extract in ıntestinal ınflammation. Mol. Nutrit. Food Res. 61: 10.
Yao, Q., Y.F. Shen, L. Bu, P. Yang, Z.P. Xu and X.Q. Guo. 2019. Ultrasound-assisted aqueous extraction of total flavonoids and hydroxytyrosol from olive leaves optimized by response surface methodology. Prep. Biochem. Biotechnol. 49 (9): 837–45.
Zoidou, E., E. Melliou, G. Moatsou and P. Magiatis. 2017. Preparation of Functional Yogurt Enriched With Olive-Derived Products. In: Yogurt in Health and Disease Prevention, p:203–220.