Journal of Environmental and Agricultural Sciences (JEAS). Özkan and Aasim, 2020. Volume 22(2): 11-19
Open Access – Research Article
Comparison of in vitro Regeneration Potential of Different Preconditioned and Nonconditioned Explants of
Peanut (Arachis hypogaea L)
Hülya Özkan 1, Muhammad Aasim 2,*
1 Department of Biology, Faculty of Science, Karamanoglu Mehmetbey University, Karaman, Turkey
2 Department of Plant Protection, Faculty of Agricultural Sciences and Technologies, Sivas University of Science and Technology, 58140 Sivas, Turkey
Abstract: The study presents the in vitro regeneration potential of three different explants of peanut. The explants used were zygotic embryos, plumular apices and the embryonic axis of peanut taken after 15 days preconditioning with 25 mg/L Benzylaminopurine (BAP) or nonconditioned on MS (Murashige and Skoog) medium without containing BAP. Subsequently, all preconditioned and nonconditioned explants were cultured on MS medium containing 1 mg/L BAP for multiple shoot induction. Both preconditioned and nonconditioned explants induced 100% shoot regeneration frequency. Shoot counts were highest from preconditioned explants, and shoot length was attributed to nonconditioned explants. Comparison of explants revealed the insignificant impact on shoot counts. However, longer shoots were achieved from zygotic embryos explants. The comparative analysis of preconditioned and nonconditioned explants revealed the highest shoot counts form preconditioned plumular apices explants (25.80) and longer shoots from nonconditioned zygotic embryo explants (4.23 cm). In vitro regenerated shoots from preconditioned and nonconditioned explants were successfully rooted, followed by successful adaptation of rooted plantlets in the pots. The results suggest that both preconditioned and nonconditioned explants of peanut can be used for in vitro regeneration.
Keywords: In vitro, regeneration, peanut, preconditioned, nonconditioned.
*Corresponding author: Muhammad Aasim: maasim@sivas.edu.tr
Cite this article as Özkan, H., M. Aasim. 2020. Comparison of in vitro regeneration potential of different preconditioned and nonconditioned explants of peanut (Arachis hypogaea L). Journal of Environmental & Agricultural Sciences. 22(2): 11-19.
Copyright © Özkan and Aasim, 2020. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium provided the original author and source are appropriately cited and credited.